7A6Z

Structure of P226G BlaC from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	53.520, 54.231, 79.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.720 - 1.300
R-factor	0.11666
Rwork	0.116
R-free	0.13737
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gdn
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.720	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	72564	3548
<I/σ(I)>	11	1.6
Completeness [%]	99.9	99.9
Redundancy	6.5
CC(1/2)	0.997	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	0.2 M Sodium malonate dibasic monohydrate, 20 % w/v PEG 3350