7A5Q
Crystal structure of VcSiaP bound to sialic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000009 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.354, 106.738, 134.506 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 1.680 |
| R-factor | 0.2144 |
| Rwork | 0.213 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b50 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.809 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.089 | 0.920 |
| Number of reflections | 152174 | 7732 |
| <I/σ(I)> | 7.1 | 1.3 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 7.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus Screen (Molecular Dimensions) condition F4 |
