7A4V
Crystal structure of lid-truncated ADP-bound BiP in an oligomeric state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.179, 51.249, 118.801 |
| Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
| Resolution | 29.270 - 1.940 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hab |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.333 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.270 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 43705 | 2937 |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 99.1 | 94.5 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.999 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2 M potassium citrate tribasic monohydrate, 20% PEG 3350 |
