7A48

Crystal structure of the APH coiled-coil in complex with Nb49

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978570
Spacegroup name	C 1 2 1
Unit cell lengths	94.580, 26.070, 54.250
Unit cell angles	90.00, 108.92, 90.00

Refinement procedure

Resolution	44.735 - 1.547
R-factor	0.1805
Rwork	0.179
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Modeller
RMSD bond length	0.006
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.735	1.603
High resolution limit [Å]	1.547	1.547
Rmeas	0.086	0.672
Number of reflections	18085	1644
<I/σ(I)>	7.61
Completeness [%]	96.9	89.83
Redundancy	3.11
CC(1/2)	0.996	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	2 M ammonium sulphate, 0.1 M sodium acetate pH 4.6