7A3Z
OSM-3 kinesin motor domain complexed with Mg.ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976411 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.890, 62.110, 45.030 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 45.030 - 2.095 |
| R-factor | 0.2128 |
| Rwork | 0.211 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b6u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.030 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmeas | 0.188 | |
| Number of reflections | 22197 | 3477 |
| <I/σ(I)> | 7.5 | 0.8 |
| Completeness [%] | 99.4 | 95 |
| Redundancy | 6.9 | 6.6 |
| CC(1/2) | 0.994 | 0.394 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.2 M KNO3 and 20% (W/V) polyethylene glycol 3350 |
