7A3S

Crystal structure of dengue 3 virus envelope glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-08-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	142.030, 142.030, 159.588
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.925 - 2.800
R-factor	0.2353
Rwork	0.233
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uzg
RMSD bond length	0.004
RMSD bond angle	0.716
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.200	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.223	2.980
Number of reflections	46309	4527
<I/σ(I)>	9.2
Completeness [%]	100.0
Redundancy	8.3	6.7
CC(1/2)	0.996	0.303

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M HEPES (7.5), 1.5 M (NH4)2SO4, 280 mM NACL