7A1N

FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II), 3-methyl-2-oxoglutarate, AND CONSENSUS ANKYRIN REPEAT DOMAIN (20-MER)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97950
Spacegroup name	P 41 21 2
Unit cell lengths	86.052, 86.052, 147.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.270 - 2.010
R-factor	0.2136
Rwork	0.212
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.002
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.370	2.044
High resolution limit [Å]	2.010	2.010
Rmerge	0.112	6.269
Number of reflections	37759	1845
<I/σ(I)>	19.8	0.6
Completeness [%]	100.0	100
Redundancy	26.3	27.1
CC(1/2)	1.000	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5