7A1F
Crystal structure of human 5' exonuclease Appollo in complex with 5'dAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.121, 54.183, 72.109 |
| Unit cell angles | 75.48, 74.33, 63.09 |
Refinement procedure
| Resolution | 43.953 - 1.800 |
| R-factor | 0.1796 |
| Rwork | 0.177 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aho |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 68.650 | 68.650 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.062 | 0.025 | 0.622 |
| Rmeas | 0.081 | 0.032 | 0.812 |
| Rpim | 0.051 | 0.020 | 0.516 |
| Total number of observations | 123105 | 1025 | 7622 |
| Number of reflections | 55714 | 448 | 3368 |
| <I/σ(I)> | 7.9 | 25.4 | 1.3 |
| Completeness [%] | 93.3 | 95.1 | 95.5 |
| Redundancy | 2.2 | 2.3 | 2.3 |
| CC(1/2) | 0.995 | 0.986 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG 3350, 0.2M Ammonium chloride |
