7A0R

50S Deinococcus radiodurans ribosome bounded with mycinamicin I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-02-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	I 2 2 2
Unit cell lengths	170.484, 408.933, 697.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.545 - 3.300
R-factor	0.2168
Rwork	0.215
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pio
RMSD bond length	0.008
RMSD bond angle	1.378
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.360
High resolution limit [Å]	3.300	8.940	3.300
Rmerge	0.182	0.119	1.145
Rmeas	0.215	0.142	1.355
Rpim	0.113	0.076	0.711
Total number of observations	1224927
Number of reflections	359719	18190	17979
<I/σ(I)>	5.5
Completeness [%]	98.4	95.8	99
Redundancy	3.4	3.4	3.3
CC(1/2)		0.960	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Ribosome solution containing 0.0065 mM (180 A/ml) of D50S in 10 mM Hepes pH=7.8, 15 mM MgCl2 and 75 mM NH4Cl crystallization buffer was mixed with 10 mM spermidine, 1 % ethanol and 0.5 % 2-ethyl-1,3-hexanediol precipitants. A 0.005 ml crystallization drop was hanged over 10 % ethanol and 5% 2-ethyl-1,3-hexanediol in ddw reservoir.