7A0J
Crystal structure of the CRINKLY WD40 ectodomain from the Arabidopsis thaliana receptor kinase ACR4
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-19 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.991870 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.991, 87.952, 88.618 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 48.050 - 1.950 |
| Rwork | 0.220 |
| R-free | 0.23910 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.050 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.125 | 1.940 |
| Number of reflections | 83857 | 13541 |
| <I/σ(I)> | 8.75 | 0.91 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 6.8 | 6.2 |
| CC(1/2) | 1.000 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 16 % PEG 6,000, 0.01 M tri-sodium citrate pH 5.0 |
