6R9I
Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 2 2 |
| 格子定数 [Å] | 91.373, 117.075, 255.319 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 86.030 - 3.000 |
| R因子 | 0.2456 |
| Rwork | 0.243 |
| R-free | 0.28950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4czw |
| Data reduction software | XDS |
| Data scaling software | xia2 (1.12) |
| Phasing software | PHASER (2.8) |
| Refinement software | PHENIX (1.14_3260) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 106.410 | 3.080 |
| 分解能 [Å] (高) | 3.000 | 3.000 |
| Rmerge_l_obs | 0.093 | 1.761 |
| 独立反射数 | 27902 | 1350 |
| <I/σ(I)> | 12.4 | 1.3 |
| 完全性 [%] | 100.0 | 99.9 |
| 冗長性 | 6.6 | 6.6 |
| CC(1/2) | 0.998 | 0.774 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.4 M ammonium phosphate |
