6Q8J
Nterminal domain of human SMU1 in complex with LSP641
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 65 2 2 |
| 格子定数 [Å] | 72.193, 72.193, 183.164 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 43.720 - 1.800 |
| R因子 | 0.16359 |
| Rwork | 0.161 |
| R-free | 0.22007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6q8f |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.457 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 44.000 | 1.880 |
| 分解能 [Å] (高) | 1.800 | 1.800 |
| Rmeas | 0.051 | 0.762 |
| 独立反射数 | 26383 | 3140 |
| <I/σ(I)> | 16.86 | 2.2 |
| 完全性 [%] | 97.4 | 97.7 |
| 冗長性 | 5.27 | 5.32 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M Bis-Tris pH 6, 16-20 % PEG 10K, 0.2 M ammonium acetate |
