6OVW
Crystal structure of ornithine carbamoyltransferase from Salmonella enterica
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-13 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 43 3 2 |
| 格子定数 [Å] | 139.214, 139.214, 139.214 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 41.975 - 1.903 |
| R因子 | 0.1678 |
| Rwork | 0.167 |
| R-free | 0.18620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1duv |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.15.2_3472) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 1.930 |
| 分解能 [Å] (高) | 1.900 | 5.160 | 1.900 |
| Rmerge_l_obs | 0.079 | 0.048 | 1.686 |
| Rmeas | 0.080 | 0.049 | 1.780 |
| Rpim | 0.014 | 0.009 | 0.560 |
| Total number of observations | 1111728 | ||
| 独立反射数 | 36844 | 2070 | 1803 |
| <I/σ(I)> | 7.3 | ||
| 完全性 [%] | 100.0 | 100 | 99.7 |
| 冗長性 | 30.2 | 29.4 | 9.7 |
| CC(1/2) | 0.999 | 0.435 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 2.0 M ammonium dihydrogen phosphate, 0.1 M Tris-Cl |
