6OM4
The structure of Microcin C7 biosynthetic enzyme MccB in complex with N-formylated MccA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-01-16 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.078 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.094, 76.059, 131.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.980 - 1.700 |
R-factor | 0.15692 |
Rwork | 0.155 |
R-free | 0.19868 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h9g |
RMSD bond length | 0.013 |
RMSD bond angle | 1.700 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.980 | 1.725 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 59837 | 4320 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 96.1 | |
Redundancy | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 282 | 0.1 M Magnesium formate, 15% w/v Polyethylene glycol 3350, and 6% w/v 1,6-Hexanediol |