6OH7
Crystal structure of (E)-biformene synthase LrdC from Streptomyces sp. strain K155 in complex with Mg
「5A0K」から置き換えられましたExperimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-18 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9790 |
Spacegroup name | P 32 2 1 |
格子定数 [Å] | 107.050, 107.050, 89.241 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 32.616 - 2.190 |
R因子 | 0.2357 |
Rwork | 0.234 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | LOW RESOLUTION SAD STRUCTURE |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 34.270 | 2.260 |
分解能 [Å] (高) | 2.190 | 2.190 |
Rmerge_l_obs | 0.092 | 1.230 |
独立反射数 | 30779 | 2617 |
<I/σ(I)> | 18.2 | 2 |
完全性 [%] | 100.0 | 100 |
冗長性 | 14.3 | 12.5 |
CC(1/2) | 0.999 | 0.726 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | MICROBATCH | 6.5 | 277 | 0.16 M MAGNESIUM ACETATE TETRAHYDRATE, 0.08 M SODIUM CACODYLATE TRIHYDRATE PH 6.5, 16% (W/V) PEG 8000, 20% (V/V) GLYCEROL |