6OGZ

In situ structure of Rotavirus RNA-dependent RNA polymerase at transcript-elongated state

EM specimen

VitrificationYES
StainingNO

EM Data Collection

MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2.7
DetectorGATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)3.6
SoftwareRELION,Leginon,,CTFFIND
SymmetryC1
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION