6O5K

Murine TRIM28 Bbox1 domain

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
格子定数 [Å]	33.012, 51.459, 72.884
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	42.040 - 1.600
R因子	0.18181
Rwork	0.180
R-free	0.20764
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.194
Data reduction software	XDS (Jan 10, 2014)
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0230)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	42.040	1.630
分解能 [Å] (高)	1.600	1.600
Rmerge_l_obs	0.267	3.950
Rpim	0.072	1.075
独立反射数	17060	817
<I/σ(I)>	10.3	0.97
完全性 [%]	100.0	100
冗長性	14.3	14.2
CC(1/2)	0.997	0.391

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION	6	291	2.7 M Ammonium sulfate, pH 6.0