6N5E
Broadly protective antibodies directed to a subdominant influenza hemagglutinin epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 31 |
| Unit cell lengths | 153.560, 153.560, 91.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.329 - 3.000 |
| R-factor | 0.257 |
| Rwork | 0.249 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1HA0 6n5b & 4HKX |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.329 | 50.000 | 3.300 |
| High resolution limit [Å] | 3.000 | 44.329 | 3.000 |
| Rmerge | 0.321 | 1.760 | |
| Rmeas | 0.350 | 1.912 | |
| Total number of observations | 311466 | ||
| Number of reflections | 48395 | 0 | 12035 |
| <I/σ(I)> | 7.28 | 1.36 | |
| Completeness [%] | 99.8 | 99.6 | |
| Redundancy | 6.436 | 6.482 | |
| CC(1/2) | 0.984 | 0.315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2 M ammonium sulfate, 0.1 M MES, pH 6.5, 20% w/v PEG8000 |
