6J7M
Complex structure of the Pseudomonas aeruginosa rhamnosyltransferase EarP with the acceptor elongation factor EF-P
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.988 |
Spacegroup name | C 1 2 1 |
格子定数 [Å] | 223.452, 56.736, 132.419 |
格子定数 [度] | 90.00, 125.03, 90.00 |
精密化法
残基 | 38.041 - 2.301 |
R因子 | 0.1907 |
Rwork | 0.188 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j7j |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 40.000 | 40.000 | 2.380 |
分解能 [Å] (高) | 2.300 | 4.950 | 2.300 |
Rmerge_l_obs | 0.113 | 0.072 | 0.468 |
Rmeas | 0.130 | 0.083 | 0.541 |
Rpim | 0.063 | 0.040 | 0.266 |
Total number of observations | 231937 | ||
独立反射数 | 59438 | 6121 | 5872 |
<I/σ(I)> | 4.8 | ||
完全性 [%] | 98.6 | 98.1 | 98 |
冗長性 | 3.9 | 4 | 3.8 |
CC(1/2) | 0.992 | 0.822 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Ammonium sulfate, 0.1 M MES pH 6.5, 20% PEG8K |