6I8X

As-p18, an extracellular fatty acid binding protein

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95369
Spacegroup name	I 4
格子定数 [Å]	102.254, 102.254, 103.392
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	14.410 - 2.300
R因子	0.181
Rwork	0.179
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g74
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.070
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.060	2.420
分解能 [Å] (高)	2.300	2.300
Rmeas	0.104	0.679
Rpim	0.049	0.435
独立反射数	23648
<I/σ(I)>	9.8	1.8
完全性 [%]	93.2	77.1
冗長性	3.7	1.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	40% ethylene glycol, 0.1 M acetate pH 4.5