6HY7
Crystal structure of alpha9 nAChR extracellular domain in complex with alpha-conotoxin RgIA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 63.646, 82.530, 49.474 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 42.434 - 2.260 |
| R因子 | 0.1962 |
| Rwork | 0.193 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uxu |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.053 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 42.434 | 2.360 |
| 分解能 [Å] (高) | 2.260 | 2.260 |
| 独立反射数 | 12622 | |
| <I/σ(I)> | 1.19 | |
| 完全性 [%] | 98.9 | |
| 冗長性 | 5.26 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 30% PEG 10000, 100mM Hepes |
