6HT1
Crystal structure of MLLT1 (ENL) YEATS domain in complexed with SGC-iMLLT (compound 92)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 48.842, 48.842, 133.238 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.860 - 2.100 |
R-factor | 0.20296 |
Rwork | 0.200 |
R-free | 0.25204 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j9s |
RMSD bond length | 0.015 |
RMSD bond angle | 1.501 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.840 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.069 | 0.463 |
Rpim | 0.027 | 0.185 |
Number of reflections | 10098 | 956 |
<I/σ(I)> | 13.2 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.8 | 7.6 |
CC(1/2) | 0.999 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 25% PEG3350, 0.2M ammonium sulfate, 0.1M bis-tris pH 5.5 or 25% PEG Smear Medi-um, 0.1M citrate pH 5.5 |