6HQB
Monomeric cyanobacterial photosystem I
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 82 |
| Detector technology | PIXEL |
| Collection date | 2017-09-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 124.322, 178.658, 181.446 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 49.289 - 4.000 |
| R因子 | 0.256 |
| Rwork | 0.255 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.299 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3228: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 |
| 分解能 [Å] (高) | 4.000 | 4.000 |
| Rpim | 0.092 | 0.437 |
| 独立反射数 | 34852 | |
| <I/σ(I)> | 4.9 | |
| 完全性 [%] | 99.7 | |
| 冗長性 | 24 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | KCl, MES-NaOH, PEG 3350, sulfoquinovosyldiacylglycerol or Jeffamine M-600 | |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | KCl, MES-NaOH, PEG 3350, sulfoquinovosyldiacylglycerol or Jeffamine M-600 |
