6H7B

Structure of Leishmania PABP1 (domain J) complexed with a peptide containing the PAM2 motif of eIF4E4.

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 65
格子定数 [Å]	83.330, 83.330, 58.160
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	36.083 - 1.890
R因子	0.1989
Rwork	0.197
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h7a
結合長の平均二乗偏差(RMSD) [Å]	0.004
結合角の平均二乗偏差(RMSD) [度]	0.583
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	36.100	1.958
分解能 [Å] (高)	1.890	1.890
Rmerge_l_obs	0.057	1.500
Rmeas	0.060	1.607
Rpim	0.019	0.574
独立反射数	18507	1829
<I/σ(I)>	18.54
完全性 [%]	99.9	99.84
冗長性	9.8	7.8
CC(1/2)	0.999	0.586

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		295	0.2M ammonium sulphate; 0.1M MES; 20% PEG 8000