6H15
Structure of LRP6 P3E3P4E4 in complex with VHH L-P2-B10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-03 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 65 |
| Unit cell lengths | 118.314, 118.314, 249.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.806 - 2.600 |
| R-factor | 0.2008 |
| Rwork | 0.198 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0p |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13rc1_2958) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 249.950 | 249.950 | 2.670 |
| High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
| Rmerge | 0.219 | 0.106 | 1.004 |
| Rmeas | 0.232 | 0.112 | 1.100 |
| Rpim | 0.074 | 0.035 | 0.433 |
| Total number of observations | 556037 | 6940 | 24042 |
| Number of reflections | 60113 | 717 | 3987 |
| <I/σ(I)> | 6.8 | 14 | 1.4 |
| Completeness [%] | 99.0 | 100 | 89.6 |
| Redundancy | 9.2 | 9.7 | 6 |
| CC(1/2) | 0.989 | 0.995 | 0.474 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M sodium citrate, 0.2 M sodium acetate trihydrate pH 5.5, 10 % PEG w/v 4000 |
