6GOY
Structure of mEos4b in the green fluorescent state
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 38.600, 58.090, 103.270 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 38.593 - 1.650 |
| R因子 | 0.1693 |
| Rwork | 0.168 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zux |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.786 |
| Data reduction software | XDS (VERSION Jun 1, 2017) |
| Data scaling software | XSCALE (VERSION Jun 1, 2017) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 38.593 | 38.593 | 1.690 |
| 分解能 [Å] (高) | 1.650 | 7.380 | 1.650 |
| Rmerge_l_obs | 0.100 | 0.066 | 0.507 |
| Rmeas | 0.121 | 0.080 | 0.625 |
| 独立反射数 | 27933 | 328 | 2083 |
| <I/σ(I)> | 6.59 | 13.8 | 1.82 |
| 完全性 [%] | 97.2 | 85.9 | 99 |
| 冗長性 | 2.753 | 2.762 | 2.741 |
| CC(1/2) | 0.991 | 0.987 | 0.699 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES, 30 % PEG 1000 |
