6GF6
Molecular basis of egg coat filament cross-linking: high-resolution structure of the partially deglycosylated ZP1 ZP-N1 domain homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.7710 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 75.980, 75.980, 101.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.966 - 2.300 |
R-factor | 0.1958 |
Rwork | 0.192 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Partially refined model derived from SAD phasing of a gold derivative |
RMSD bond length | 0.005 |
RMSD bond angle | 0.738 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_3409: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.990 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.116 | 1.498 |
Rpim | 0.026 | 0.495 |
Number of reflections | 13550 | 1422 |
<I/σ(I)> | 22.2 | 1.4 |
Completeness [%] | 98.0 | 84.6 |
Redundancy | 19.7 | 8.3 |
CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.6 M LiCl, 0.1 M tri-sodium citrate pH 5.5 |