6DFS
mouse TCR I.29 in complex with IAg7-p8E9E6ss
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 63 |
| 格子定数 [Å] | 269.518, 269.518, 45.639 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 50.010 - 3.100 |
| R因子 | 0.24197 |
| Rwork | 0.240 |
| R-free | 0.28515 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.096 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 3.300 |
| 分解能 [Å] (高) | 3.100 | 3.100 |
| 独立反射数 | 32259 | |
| <I/σ(I)> | 24.4 | |
| 完全性 [%] | 99.7 | |
| 冗長性 | 6.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 12% PEG 20000, 100mM bicine pH9.0, 20% ethylene glycol |
