6D5G

Ras:SOS:Ras in complex with a small molecule activator

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98
Spacegroup name	I 4 2 2
格子定数 [Å]	183.050, 183.050, 178.784
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	48.838 - 1.920
R因子	0.1561
Rwork	0.155
R-free	0.17250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nyi
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	0.871
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.950
分解能 [Å] (高)	1.920	1.920
Rmerge_l_obs		0.215
Rpim	0.029
独立反射数	114822	5674
<I/σ(I)>	27.8
完全性 [%]	100.0
冗長性	16.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0