6CFI
Crystal structure of Rad4-Rad23 bound to a 6-4 photoproduct UV lesion
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 18-ID |
| Synchrotron site | APS |
| Beamline | 18-ID |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2010-10-28 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 80.201, 80.201, 405.025 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 74.566 - 3.362 |
| R因子 | 0.221283344424 |
| Rwork | 0.219 |
| R-free | 0.26822 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12rc1_2801) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 68.950 | 3.483 |
| 分解能 [Å] (高) | 3.362 | 3.362 |
| Rmerge_l_obs | 0.232 | |
| Rmeas | 0.241 | |
| Rpim | 0.064 | |
| 独立反射数 | 19112 | 1407 |
| <I/σ(I)> | 11.98 | |
| 完全性 [%] | 95.9 | 72.75 |
| 冗長性 | 13.8 | 8.2 |
| CC(1/2) | 0.998 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | bis-tris propane 5 mM sodium chloride 100 mM 1-propanol 14% spermidine-HCl 5 mM dithiothreitol 5 mM |
