6ABM
Crystal structure of Methanosarcina mazei PylRS(Y306A/Y384F) complexed with pTmdZLys
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-22 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.604, 44.209, 72.553 |
Unit cell angles | 90.00, 118.48, 90.00 |
Refinement procedure
Resolution | 35.024 - 1.368 |
R-factor | 0.1635 |
Rwork | 0.162 |
R-free | 0.19870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zim 2zce |
RMSD bond length | 0.008 |
RMSD bond angle | 1.112 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.400 |
High resolution limit [Å] | 1.368 | 1.370 |
Rmeas | 0.166 | |
Rpim | 0.075 | 0.304 |
Number of reflections | 59383 | 2804 |
<I/σ(I)> | 15.1 | |
Completeness [%] | 99.6 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | Tris-HCl buffer (pH 8.5), PEG 200, KCl, MgCl2, sodium citrate |