6A6L
Crystal structure of the cold shock domain of YB-1 in complex with m5C RNA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 62 |
| 格子定数 [Å] | 66.380, 66.380, 34.702 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 29.709 - 1.780 |
| R因子 | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pf5 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.934 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 1.840 |
| 分解能 [Å] (高) | 1.780 | 1.780 |
| Rmerge_l_obs | 0.070 | 0.191 |
| 独立反射数 | 8518 | 856 |
| <I/σ(I)> | 67.973 | |
| 完全性 [%] | 100.0 | |
| 冗長性 | 17.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2.4M Ammonium sulfate |
