6A5G
The structure of [4+2] and [6+4] cyclase in the biosynthetic pathway of streptoseomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 52.109, 85.023, 134.797 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.604 - 2.300 |
R-factor | 0.1849 |
Rwork | 0.180 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a5f |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.604 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.199 | |
Number of reflections | 24828 | |
<I/σ(I)> | 20.93 | |
Completeness [%] | 90.7 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295.15 | 2 M ammonium sulfate, 100 mM MES, pH 6.5, 200mM NaCl |