6ZZM

Crystal structure of the catalytic domain of Corynebacterium mustelae predicted acetyltransferase AceF (E2p).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-09
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966000
Spacegroup name	P 63 2 2
Unit cell lengths	100.619, 100.619, 281.568
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.380 - 2.500
R-factor	0.2016
Rwork	0.201
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zzi
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	autoPROC
Data scaling software	autoPROC (1.0.5)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.380	2.600
High resolution limit [Å]	2.500	2.500
Rpim	0.028	0.487
Number of reflections	30240	3318
<I/σ(I)>	18.7	1.7
Completeness [%]	100.0	100
Redundancy	19	19.6
CC(1/2)	0.999	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Hepes-Na pH 7.5, 150 mM NaCl, 30% PEG4000