6ZZL

Crystal structure of the catalytic domain plus N-terminal linker of the acetyltransferase AceF (E2p) from Corynebacterium glutamicum.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 64
Unit cell lengths	170.392, 170.392, 67.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.196 - 2.229
R-factor	0.1915
Rwork	0.190
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zzj
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	autoPROC
Data scaling software	autoPROC (1.0.5)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.196	2.371
High resolution limit [Å]	2.229	2.229
Rpim	0.027	0.484
Number of reflections	40013	2001
<I/σ(I)>	16	1.5
Completeness [%]	95.0	74.8
Redundancy	20.6	15.9
CC(1/2)	0.999	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	30% PEG1500