6ZZB

Crystal structure of the Eimeria tenella surface antigen protein SAG19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	I 4
Unit cell lengths	108.120, 108.120, 37.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.400 - 1.322
Rwork	0.165
R-free	0.18760
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.596
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.411	1.340
High resolution limit [Å]	1.320	1.320
Rmerge	0.036	0.579
Rpim	0.024	0.484
Number of reflections	49236	2869
<I/σ(I)>	17.6	1.4
Completeness [%]	96.9
Redundancy	5.5
CC(1/2)	0.999	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	3.15 M ammonium sulfate, 0.1 M citrate buffer pH 5