6ZYT
Monomeric streptavidin with a conjugated biotinylated pyrrolidine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976220 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.790, 52.740, 89.360 |
| Unit cell angles | 75.24, 78.24, 84.88 |
Refinement procedure
| Resolution | 25.480 - 1.800 |
| Rwork | 0.259 |
| R-free | 0.30080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jnj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.673 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.480 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 64049 | 3764 |
| <I/σ(I)> | 5.6 | 1.6 |
| Completeness [%] | 95.7 | |
| Redundancy | 1.7 | |
| CC(1/2) | 0.967 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.2 M ammonium sulfate, 0.1 M sodium cacodylate, 30% w/v PEG 8000 |
