6ZXZ

Sarcin-Ricin Loop RNA from Ecoli with a A2670-2'-OCF3 modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	62.412, 75.267, 52.981
Unit cell angles	90.00, 105.60, 90.00

Refinement procedure

Resolution	46.970 - 2.440
R-factor	0.212
Rwork	0.211
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.002
RMSD bond angle	0.531
Data reduction software	XDS (31 Jan 2020)
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.440	2.440
Number of reflections	17179	2748
<I/σ(I)>	6.23
Completeness [%]	99.5
Redundancy	3.5
CC(1/2)	0.990	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	RNA 1 volume Buffer (NaCl 80 mM, KCl 12 mM, MgCl2 20 mM, NaCaco pH7.0 40 mM, MPD 35 %, spermine tetrahydrochloride 12 mM) 1 volume