6ZXY

Structure of Archaeoglobus fulgidus Trm11 m2G10 tRNA methyltransferase enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	73.134, 73.134, 155.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.060 - 2.750
R-factor	0.202
Rwork	0.199
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zxv
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.910
High resolution limit [Å]	2.750	2.750
Rmerge	0.146
Number of reflections	11597	1808
<I/σ(I)>	12.2	1.08
Completeness [%]	99.7
Redundancy	8.6
CC(1/2)	0.998	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	297	0.1 M Sodium citrate pH 6, 18% PEG 4k, 20% isopropanol