6ZXW

Structure of Archaeoglobus fulgidus Trm11-Trm112 m2G10 tRNA methyltransferase complex bound to sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	75.360, 92.940, 101.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.550 - 2.190
R-factor	0.199
Rwork	0.198
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zxv
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.550	2.330
High resolution limit [Å]	2.190	2.190
Rmerge	0.065	1.231
Number of reflections	36999	5852
<I/σ(I)>	13.1	1.1
Completeness [%]	99.6	98.9
Redundancy	4.5
CC(1/2)	0.998	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	297	0.1 M Sodium citrate pH 6; 9% PEG 4000, 0.1 M ammonium sulfate