6ZXT
High resolution crystal structure of chloroplastic ribose-5-phosphate isomerase from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.152, 63.952, 80.909 |
| Unit cell angles | 90.00, 96.13, 90.00 |
Refinement procedure
| Resolution | 44.890 - 1.400 |
| R-factor | 0.1581 |
| Rwork | 0.158 |
| R-free | 0.17640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mc0 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.890 | 1.454 |
| High resolution limit [Å] | 1.400 | 1.404 |
| Rmerge | 0.052 | 1.272 |
| Rmeas | 0.056 | |
| Rpim | 0.021 | |
| Number of reflections | 89012 | 8611 |
| <I/σ(I)> | 20.58 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.999 | 0.382 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 2 | EVAPORATION | 293.15 | ammonium sulfate, glycerol |
