6ZXB

Diguanylate cyclase DgcR (I-site mutant) in native state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-09-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.979389
Spacegroup name	C 1 2 1
Unit cell lengths	136.676, 39.344, 146.222
Unit cell angles	90.00, 108.77, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.2282
Rwork	0.227
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v0n
RMSD bond length	0.012
RMSD bond angle	1.661
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.040	2.250
High resolution limit [Å]	2.190	2.190
Rmerge	0.099	1.328
Rmeas	0.111
Rpim	0.048	0.917
Number of reflections	38136	3199
<I/σ(I)>	10.7
Completeness [%]	98.4
Redundancy	5	5
CC(1/2)	0.998	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	0.2 M Magnesium sulfate, 20%PEG 3350