6ZWW

Crystal structure of E. coli RNA helicase HrpA in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	C 1 2 1
Unit cell lengths	220.854, 126.198, 179.253
Unit cell angles	90.00, 110.76, 90.00

Refinement procedure

Resolution	49.400 - 3.160
R-factor	0.2267
Rwork	0.226
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i3p
RMSD bond length	0.004
RMSD bond angle	0.763
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	3.340
High resolution limit [Å]	3.150	3.150
Number of reflections	78441	12390
<I/σ(I)>	8.94	0.49
Completeness [%]	99.0
Redundancy	5
CC(1/2)	0.998	0.224

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	8% [w/v] PEG 6000, 0.2 M calcium chloride, 0.1 M MES pH 6