6ZVE

14-3-3 Sigma in complex with phosphorylated Gab2pT391 peptide - 1h incubation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-16
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541
Spacegroup name	C 2 2 21
Unit cell lengths	82.896, 113.033, 62.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.420 - 2.510
R-factor	0.2472
Rwork	0.246
R-free	0.26883
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lw1
RMSD bond length	0.009
RMSD bond angle	1.289
Refinement software	REFMAC (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.330	2.500
High resolution limit [Å]	2.500	2.500
Number of reflections	10057	723
<I/σ(I)>	4
Completeness [%]	96.4
Redundancy	5.5
CC(1/2)	0.891	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG400, 1.25% glycerol, 0.2M CaCl, 0.1M HEPES pH 7.5, 2mM BME