6ZUE

Crystal structure of human DDB1 bound to human DCAF1 (amino acid residues 1046-1396)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.92819
Spacegroup name	I 2 2 2
Unit cell lengths	117.384, 153.626, 223.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.634 - 3.094
R-factor	0.2249
Rwork	0.222
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e0c
RMSD bond length	0.004
RMSD bond angle	0.759
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.280
High resolution limit [Å]	3.090	3.090
Number of reflections	36027	5769
<I/σ(I)>	12.98
Completeness [%]	96.8
Redundancy	4.82
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	100 mM Tri-Na citrate pH 5.5 18% PEG 1000