6ZUA
Cu nitrite reductase from Achromobacter cycloclastes: MSOX series at 170K, dose point 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-07-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.7 |
Spacegroup name | P 21 3 |
Unit cell lengths | 95.350, 95.350, 95.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.680 - 1.140 |
R-factor | 0.1236 |
Rwork | 0.122 |
R-free | 0.15620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bw4 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.680 | 1.200 |
High resolution limit [Å] | 1.140 | 1.140 |
Rpim | 0.036 | 0.295 |
Number of reflections | 104229 | 15193 |
<I/σ(I)> | 9.1 | 2.2 |
Completeness [%] | 99.5 | 100 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.6M AMMONIUM SULPHATE, 100MM REMARK 280 SODIUM ACETATE, PH 4.75 |