6ZU6
Cu nitrite reductase from Achromobacter cycloclastes: MSOX series at 170K, dose point 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 95.340, 95.340, 95.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.950 - 1.150 |
| R-factor | 0.1202 |
| Rwork | 0.118 |
| R-free | 0.15910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bw4 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.210 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rpim | 0.041 | 0.310 |
| Number of reflections | 101516 | 14815 |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 99.5 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 293 | Crystals were grown using the vapor diffusion hanging drop method with a protein concentration of 10 mg/ml. The reservoir solution consisted of 1.6 M ammonium sulfate and 0.1 M sodium acetate (pH 4.75). |
