6ZT4
Pentapeptide repeat protein MfpA from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.006, 85.731, 66.336 |
| Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
| Resolution | 52.490 - 1.770 |
| R-factor | 0.2092 |
| Rwork | 0.208 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bm4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.502 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.280 | 66.280 | 1.810 |
| High resolution limit [Å] | 1.770 | 9.030 | 1.770 |
| Rmerge | 0.097 | 0.044 | 1.517 |
| Rmeas | 0.106 | 0.048 | 1.729 |
| Rpim | 0.041 | 0.018 | 0.809 |
| Total number of observations | 1721 | 8389 | |
| Number of reflections | 33772 | 273 | 1901 |
| <I/σ(I)> | 8.9 | 27.4 | 0.9 |
| Completeness [%] | 99.8 | 99.6 | 97.1 |
| Redundancy | 6.3 | 6.3 | 4.4 |
| CC(1/2) | 0.999 | 0.999 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | NULL |
