6ZT3
N-terminal 47 kDa fragment of the Mycobacterium smegmatis DNA Gyrase B subunit complexed with ADPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 76.985, 76.985, 261.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.690 - 1.560 |
| R-factor | 0.1493 |
| Rwork | 0.147 |
| R-free | 0.19390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zkb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.368 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.82) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.370 | 65.370 | 1.590 |
| High resolution limit [Å] | 1.560 | 8.540 | 1.560 |
| Rmerge | 0.141 | 0.034 | 2.486 |
| Rmeas | 0.143 | 0.035 | 2.518 |
| Rpim | 0.023 | 0.006 | 0.399 |
| Total number of observations | 15049 | 127674 | |
| Number of reflections | 66509 | 540 | 3231 |
| <I/σ(I)> | 16.7 | 62.3 | 1.9 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 38.8 | 27.9 | 39.5 |
| CC(1/2) | 1.000 | 1.000 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | NULL |
