6ZSY

Crystal structure of the Grindelwald extracellular domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.978
Spacegroup name	P 41 21 2
Unit cell lengths	31.483, 31.483, 97.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.967 - 0.926
Rwork	0.130
R-free	0.15710
Structure solution method	SAD
Data reduction software	XDS
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.967	0.942
High resolution limit [Å]	0.926	0.926
Rmerge	0.064	0.412
Number of reflections	33869	1382
<I/σ(I)>	21.5	3
Completeness [%]	98.2	83.8
Redundancy	10.6
CC(1/2)	0.999	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M Na-Hepes pH 7.5, 45 % MPD, 0.2 M Ammonium Acetate concentration 82 mg/ml